iseq15150 extra thick blot filter paper Search Results


95
Bio-Rad iseq15150 extra thick blot filter paper
Iseq15150 Extra Thick Blot Filter Paper, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Merck KGaA pvdf membrane

Pvdf Membrane, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


Journal: iScience

Article Title: Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi

doi: 10.1016/j.isci.2024.110090

Figure Lengend Snippet:

Article Snippet: Proteins were transferred to a PVDF membrane (Merck Millipore, ISEQ15150) overnight at 4°C, then membranes were blocked with 5% skimmed milk in TBS-T. For KRAB-dCas9 detection proteins were stained with an anti-HA (1:1,000; Cell Signaling Technology 3724, RRID: AB_1549585 ), or anti-beta-Actin (1:1,000; Cell Signaling Technology 3700, RRID: AB_2242334 ) primary antibody for 2 h at RT.

Techniques: Virus, Recombinant, Membrane, Lysis, CRISPR, DNA Extraction, Western Blot, Transfection, Reverse Transcription, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Stripping, Software, Microscopy